Dr. Dechen Jiang is currently a professor in State Key Laboratory of Analytical Chemistry for Life, School of Chemistry and Chemical Engineering at Nanjing University.He obtained his B.S. M.S and Ph.D. degrees from Nanjing University (2000), Fudan University (2003) and Case Western Reserve University (2008).After three years postdoctoral training at UNC-Chapel Hill, Dr. Jiang joined Nanjing University to start his independent research.Dr. Jiang has authored and coauthored over 50 research publications such as PNAS, JACS, Angew Chem and Anal. Chem.His research focuses on the development of electrochemical methodology and instrumental for the characterization of cellular activity and material features.He has received multiple awards, such as Changjiang Young Professor of the Ministry of Education （2018-2021）, Young Innovation Award for Analytical Instrument (China Instrument and Control Society).

Our group focuses on the development of more intricate electrochemical devices for the analysis of molecules in single cells, and even in individual intracellular compartments.  The achievement of these methods, and even devices, would advance the knowledge of cellular heterogeneity and protein function at subcellular locations.  

(1) Nanokit for single cell analysis.  

Our group developed nano-capillary electrodes kits, named as “Nanokits”, for cost-effective analysis of enzyme activity within single living cells and even at single lysosomes.  The nanokit that is special for the target enzyme is assembled in a nanometer-sized capillary with a working electrode, and electrochemically loaded into the cell allowing the electrochemical quantification of the enzyme activity.  Furthermore, the enzyme activity in individual intracellular compartments can be characterized by reversed electrochemical pumping to confine the targeted organelle in the nano-capillary tip with the nanokit.  The use of commercially available reagent kits marketed for cell population studies permits direct application of the nano-capillary electrode for targeting a variety of enzymes in single cells. 

Recent work:

1. Pan R, Xu M, Jiang D*, Burgess JD, Chen HY. Nanokit for single-cell electrochemical analyses. Proc Natl Acad Sci USA. 2016, 113(41):11436-11440.

2. Pan R, Xu M, Burgess JD, Jiang D*, Chen HY. Direct electrochemical observation of glucosidase activity in isolated single lysosomes from a living cell. Proc Natl Acad Sci USA. 2018, 115(16):4087-4092.

3. Pan R, Hu K, Jiang D*, Samuni U, Mirkin MV*. Electrochemical Resistive-Pulse Sensing. J Am Chem Soc. 2019, 141(50):19555-19559.

(2) ECL imaging for high-throughput single cell analysis

High-throughput and spatial/temporal analysis of single living cells provides valuable information about the cell features that are associated with cellular heterogeneity. Although fluorescence imaging is popular for investigating the distribution and content of biomolecules, the design and synthesis of specific fluorescence probes for small molecules are time consuming, and the specificities of these probes are often subject to caution. Our lab used commercially available oxidases to react with the corresponding small molecules at the cells generating hydrogen peroxide, which produces enhanced electrochemiluminescence (ECL) from aqueous luminol for single cell analysis.  Currently, we have applied this strategy and the developed ECL microscopy to high-throughput bioanalysis and to visualize the distribution of molecules at single cells. Moreover, this ECL imaging could efficiently minimize the background signals for the sensitive measurement of small molecules and membrane proteins at cells.

Recent work: 

1. Zhang J, Jin R, Jiang D*, Chen HY. Electrochemiluminescence-Based Capacitance Microscopy for Label-Free Imaging of Antigens on the Cellular Plasma Membrane. J Am Chem Soc. 2019, 141(26):10294-10299.

2. Zhang J, Arbault S, Sojic N*, Jiang D*. Electrochemiluminescence Imaging for Bioanalysis. Annu Rev Anal Chem 2019, 12;12(1):275-295.

3. Jin R, Huang YC, Chen L, Lu HY, Jiang D*, Chen HY. In situ observation of heterogeneous charge distribution at the electrode unraveling the mechanism of electric field-enhanced electrochemical activity. Chem. Sci. 2020, 11, 4158-4163. 

4. Wang Y, Jin R, Sojic N*, Jiang D*, Chen HY. Intracellular Wireless Analysis of Single Cells by Bipolar Electrochemiluminescence Confined in a Nanopipette. Angew Chem Int Ed Engl. 2020 doi: 10.1002/anie.202002323.

98. Pan RR*, Wang DC, Liu K, Chen HY, Jiang DC*. Electrochemical molecule trap-based sensing of low-abundance enzymes in one living cell. J. Am. Chem. Soc. 2022, 144, 17558-17566.

97. Liu K, Liu RJ, Wang DC, Pan RR*, Chen HY, Jiang DC*. Spatial analysis of reactive oxygen species in a 3D cell model using a sensitive nanocavity electrode. Anal. Chem. 2022, 94, 13287-13292.

96. Yang F, Jin R, Jiang DC*. High spatial resolution imaging of the charge injection yield at hematite using scanning electrochemical cell microscopy. Electrochem. commun. 2022, 141, 107358.

95. Dong XR, Huang YC, Jiang DC*. Fluorescent polymerase chain reaction nanokit for the detection of DNA sequence in single living cells. Anal. Chem. 2022, 94, 10304-10307.

94. Chang XQ, Wang NN, Jiang DP*, Chen HY, Jiang DC*. Nanokit coupled electrospray ionization mass spectrometry for analysis of angiotensin converting enzyme 2 activity in single living cell. Chin. Chem. Lett. 2022, DOI: 10.1016/j.cclet.2022.05.036

93. Liu YL, Fan JH, Zhang N, Xu HY, Su W, Qin Y, Jiang DC*. Trihexyltetradecylphosphonium chloride based ratiometric fluorescent nanosensors for multiplex anion discrimination. Analyst 2022, 147, 3209-3218.

92. Knežević S, Bouffier L, Liu BH*, Jiang DC*, Sojic N*. Electrochemiluminescence microscopy: from single objects to living cells. Curr. Opin. Electrochem. 2022, 35, 101096.

91. Li Y, Jin R, Xu LF, Jiang DP*, Chen HY, Jiang DC*. Electrochemically imaging the response of ion-selective membranes with an ultralow detection limit. ACS Appl. Mater. Interfaces 2022, 14, 14097-14102.

90. Li DR, Wang NN, Zhang TY, Wu GX, Xiong YF, Du QQ, Tian YF, Zhao WW, Ye JD, Gu SL, Lu YQ, Jiang DC*, Xu F*. Label-free fiber nanograting sensor for real-time in situ early monitoring of cellular apoptosis. Adv. Photonics 2022, 4, 016001.

89. Xu LF, Li Y, Jin R, Jiang DP*, Jiang DC*. High spatial resolution observation of Temporin A at cell membranes using scanning ion conductive microscopy. Electrochem. commun. 2022, 134, 107181.

88. Cheng L, Jin R, Jiang DC*, Zhuang J*, Liao XB, Zheng QQ. Scanning electrochemical cell microscopy platform with local electrochemical impedance spectroscopy. Anal. Chem. 2021, 93, 16401-16408.

87. Zhu H, Jin R, Chang YC, Zhu JJ, Jiang DC, Lin YH*, Zhu WL*. Understanding the synergistic oxidation in dichalcogenides through electrochemiluminescence blinking at millisecond resolution. Adv. Mater. 2021, 33, 2105039.

86. Liu YJ, Zhang HD, Li BX, Liu JW, Jiang DC*, Liu BH*, Sojic N*. Single biomolecule imaging by electrochemiluminescence. J. Am. Chem. Soc.2021, 143, 17910-17914.

85. Xu YY, Liu K, Jin R, Jiang DC*, Fang DJ*. Dynamic visualization of free radicals at single oxygen bubble using chemiluminescence. Chem. Asian J. 2021, 16, 4049-4052.

84. Wang NN, Pan RR, Ji LN*, Jiang DC*, Chen HY. Photoelectrochemical analysis of the alkaline phosphatase activity in single living cells. Analyst 2021, 146, 5528-5532.

83. Wang NN, Wang DN, Pan RR, Wang DC*, Jiang DC*, Chen HY. Self-referenced nanopipette for electrochemical analysis of hydrogen peroxide in the nucleus of a single living cell. Anal. Chem. 2021, 93, 10744-10749.

82. Jin R, Lu Hy, Cheng L, Zhuang J, Jiang DC*, Chen HY. Highly spatial imaging of electrochemical activity on the wrinkles of graphene using all-solid scanning electrochemical cell microscopy. Fundam. Res. 2022, 2, 193-197.

81. Wang YL, Pan RR, Jiang DP*, Jiang DC*, Chen HY. Nanopipettes for the electrochemical study of enhanced enzymatic activity in a femtoliter space. Anal. Chem. 2021, 93, 14521-14526.

80. Wang YL, Jiang DC*, Chen HY. Wireless electrochemical-visualization of intracellular antigens at single cells. CCS Chem. 2021, 3, 2268-2274.

79. Chen Y, Gou XD, Ma C*, Jiang DC*, Zhu JJ. A synergistic coreactant for single-cell electrochemiluminescence imaging: guanine-rich ssDNA-Loaded high-index faceted gold nanoflowers. Anal. Chem. 2021, 93, 7682-7689.

78. Jin R, Cheng L, Lu HY, Zhuang J*, Jiang DC*, Chen HY. High spatial resolution electrochemical microscopic observation of enhanced charging under bias at active sites of N-rGO. ACS Appl. Energy Mater. 2021, 4, 3502-3507.

77. Zhu H, Jiang DC*, Zhu JJ*. High-resolution imaging of catalytic activity of a single graphene sheet using electrochemiluminescence microscopy. Chem. Sci. 2021, 12, 4794-4799.

76. Cui C, Jin R, Jiang DC*, Zhang JR*, Zhu JJ*. Visualization of an accelerated electrochemical reaction under an enhanced electric field. Research 2021, 2021, 1-9.

75. Zhang JJ, Jin R, Chen Y, Fang DJ*, Jiang DC*. Enhanced electrochemiluminescence at single lithium iron phosphate nanoparticles for the local sensing of hydrogen peroxide efflux from single living cell under a low voltage. Sens. Actuators B Chem. 2021, 329, 129208.

74. Huang RC, Jin R, Jiang DC*, Chen HY. Single-cell-resolved measurement of enzyme activity at the tissue level using drop-on-demand microkits. Analyst 2021, 146, 1548-1551.

73. Qian YZ, Li Y, Qin Y, Jiang DC*, Chen HY. Ion-selective polymer dots for photoelectrochemical detection of potassium ions. Analyst 2021, 146, 450-453.

72. Wang YL, Jiang DC*, Chen HY. Electrochemiluminescence analysis of hydrogen peroxide using L012 modified electrodes. JOAT 2020, 4, 122-127.

71. Zhang JJ, Jin R, Fang DJ*, Jiang DC*. Voltage modulated electrochemiluminescence for highly sensitive detection. Chem J. Chinese U. 2020, 41, 2421-2425.

70. Li Y, Feng JJ, Huang YC, Qin Y, Jiang DC*, Chen HY. Upconverting ion-selective nanoparticles for the imaging of intracellular calcium ions. Analyst 2020, 145, 4768-4771.

69. Liu GN, Kolodziej C, Jin R, Qi SP, Lou YB*, Chen JX, Jiang DC*, Zhao YX*, Burda C*. MoS₂-stratified CdS-Cu₂-_xS core-shell nanorods for highly-efficient photocatalytic hydrogen production. ACS Nano 2020, 14, 5468-5479.

68. Wang YL, Jin R, Sojic N*, Jiang DC*, Chen HY. Intracellular wireless analysis of single cells by bipolar electrochemiluminescence confined in a nanopipette. Angew. Chem. Int. Ed.2020, 59, 10416-10420.

67. Pan RR, Hu KK, Jia R, Rotenberg SA, Jiang DC*, Mirkin MV*. Resistive-pulse sensing inside single living cells. J. Am. Chem. Soc. 2020, 142, 5778-5784.

66. Jin R, Huang YC, Cheng L, Lu HY, Jiang DC*, Chen HY. In situ observation of heterogeneous charge distribution at the electrode unraveling the mechanism of electric field-enhanced electrochemical activity. Chem. Sci. 2020, 11, 4158-4163.

65. Cui C, Jin R, Jiang DC*, Zhang JR*, Zhu JJ*. Electrogenerated chemiluminescence in submicron wells for very-high-density biosensing. Anal. Chem. 2020, 92, 578-582.

64. Gao TB, Zhang JJ, Wen J, Yang XX, Ma HB, Cao DK*, Jiang DC*. Single-molecule microRNA electrochemiluminescence detection using cyclometalated dinuclear Ir(III) complex with synergistic effect. Anal. Chem. 2020, 92, 1268-1275.

63. Li Y, Chen FZ, Xu YT, Yu WH, Li HY*, Fan GC, Han DM, Zhao WW*, Jiang DC*. Self-assembled peptide nanostructures for photoelectrochemical bioanalysis application: a proof-of-concept study. Anal. Chem. 2019, 91, 12606-12610.

62. Pan RR, Hu KK, Jiang DC*, Samuni U, Mirkin MV*. Electrochemical resistive-pulse sensing. J. Am. Chem. Soc. 2019, 141, 19555-19559.

61. Zhu H, Jin R, Jiang DC*, Zhu JJ*. Perturbation electrochemiluminescence imaging to observe the fluctuation of charge-transfer resistance at individual graphene microsheets with redox-induced defects. ACS Appl. Mater. Interfaces 2019, 11, 46666-46670.

60. Pan RR, Jiang DC*. Nanokits for the electrochemical quantification of enzyme activity in single living cells. Meth. Enzymol. 2019, 628, 173-189.

59. Zhang JJ, Jin R, Jiang DC*, Chen HY. Electrochemiluminescence-based capacitance microscopy for label-free imaging of antigens on the cellular plasma membrane. J. Am. Chem. Soc.2019, 141, 10294-10299.

58. Xu HY, Yang DD, Jiang DC*, Chen HY. Phosphate assay kit in one cell for electrochemical detection of intracellular phosphate ions at single cells. Front. Chem. 2019, 7, 1-6.

57. Chen Y, Zhao DB, Fu JJ, Gou XD, Jiang DC*, Dong H*, Zhu JJ*. In situ imaging facet-induced spatial heterogeneity of electrocatalytic reaction activity at the subparticle level via electrochemiluminescence microscopy. Anal. Chem. 2019, 91, 6829-6835.

56. Zhang JJ, Arbault S, Sojic N*, Jiang DC*. Electrochemiluminescence imaging for bioanalysis. Annu. Rev. Anal. Chem.2019, 12, 275-295.

55. Zhang JJ, Ding H, Zhao S, Jiang DC*, Chen HY. Confined electrochemiluminescence in vertically ordered silica mesochannels for the imaging of hydrogen peroxide released from single cells. Electrochem. Commun. 2019, 98, 38-42.

54. Jin R, Ye XD, Fan JP, Jiang DC*, Chen HY. In situ imaging of photocatalytic activity at titanium dioxide nanotubes using scanning ion conductance microscopy. Anal. Chem. 2019, 91, 2605-2609.

53. Huang SH, Liu K, Jiang DC, Fang DJ*. Codetermination of sphingomyelin and cholesterol in cellular plasma membrane in sphingomyelin-depletion-induced cholesterol efflux. Anal. Chem. 2019, 91, 1501-1506.

52. Cui C, Chen Y, Jiang DC*, Chen HY, Zhang JR*, Zhu JJ*. Steady-state electrochemiluminescence at single semiconductive titanium dioxide nanoparticles for local sensing of single cells. Anal. Chem. 2019, 91, 1121-1125.

51. Tao L, Qiao M, Jin R, Li Y, Xiao ZH, Wang YQ, Zhang NN, Xie C, He QG, Jiang DC, Yu G*, Li YF*, Wang SY*. Bridging the surface charge and catalytic activity of a defective carbon electrocatalyst. Angew. Chem. Int. Ed. 2019, 58, 1019-1024.

50. Xu MC, Pan RR, Zhu Y, Jiang DC*, Chen HY*. Molecular profiling of single axons and dendrites in living neurons using electrosyringe-assisted electrospray mass spectrometry. Analyst 2019, 144, 954-960.

49. Xu MC, Pan RR, Zhu Y, Jiang DC*, Chen HY*. Resistive analysis of hydrogen peroxide in one axon of single neuron with nanopipets. Anal. Chem. 2018, 90, 10117-10121.

48. Chen Y, Fu JJ, Cui C, Jiang DC*, Chen ZX*, Chen HY, Zhu JJ*. In situ visualization of electrocatalytic reaction activity at quantum dots for water oxidation. Anal. Chem. 2018, 90, 8635-8641.

47. Zhou JY, Jiang DC*, Chen HY. Nanoelectrochemical architectures for high-spatial-resolution single cell analysis. Sci. China. Chem. 2017, 60, 1277-1284.

46. Zhou S, Peng X, Xu HY, Qin Y, Jiang DC*, Qu JL*, Chen HY. Fluorescence lifetime-resolved ion-selective nanospheres for simultaneous imaging of calcium ion in mitochondria and lysosomes. Anal. Chem. 2018, 90, 7982-7988.

45. Xia J, Zhou JY, Zhang RG, Jiang DC, Jiang DP*. Gold-coated polydimethylsiloxane microwells for high-throughput electrochemiluminescence analysis of intracellular glucose at single cells. Anal. Bioanal. Chem. 2018, 410, 4787-4792.

44. Pan RR, Xu MC, Burgess JD, Jiang DC*, Chen HY. Direct electrochemical observation of glucosidase activity in isolated single lysosomes from a living cell. Proc. Natl. Acad. Sci. U.S.A. 2018, 115, 4087-4092.

43. Gao TB, Zhang JJ, Yan RQ, Cao DK*, Jiang DC*, Ye DJ*. Aggregation-induced electrochemiluminescence from a cyclometalated iridium(III) complex. Inorg. Chem. 2018, 57, 4310-4316.

42. Xu HY, Zhou S, Jiang DC*, Chen HY. Cholesterol oxidase/triton X-100 parked microelectrodes for the detection of cholesterol in plasma membrane at single cells. Anal. Chem. 2018, 90, 1054-1058.

41. Zhang JJ, Zhou JY, Pan RR, Jiang DC*, Burgess JD, Chen HY. New frontiers and challenges for single-cell electrochemical analysis. ACS Sens. 2018, 3, 242-250.

40. Li Y, Zhang N, Zhao WW*, Jiang DC*, Xu JJ, Chen HY. Polymer dots for photoelectrochemical bioanalysis. Anal. Chem. 2017, 89, 4945-4950.

39. Zhang JJ, Zhou JY, Tian CX, Yang S, Jiang DC*, Zhang XX*, Chen HY. Localized electrochemiluminescence from nanoneedle electrodes for very-high-density electrochemical sensing. Anal. Chem. 2017, 89, 11399-11404.

38. Xu FF, Zhou WX, Cao JX, Xu QQ, Jiang DC, Chen Y*. A combination of DNA-peptide probes and liquid chromatography-tandem mass spectrometry (LC-MS/MS): a quasi-targeted proteomics approach for multiplexed microRNA quantification. Theranostics 2017, 7, 2849-2862.

37. Li L, Lu BY, Jiang DC, Shin M, Kelley TJ, Burgess JD*. Cell plasma membrane cholesterol as a diagnostic. Curr. Opin. Electrochem. 2017, 2, 82-87.

36. Zhang XX*, Han FF, Syed A, Bukhari EM, Siang BCJ, Yang S, Zhou BP, Wen WJ, Jiang DC*. Fabrication of highly modulable fibrous 3D extracellular microenvironments. Biomed. Microdevices 2017, 19, 1-12.

35. Cui C, Chen Y, Jiang DC*, Zhu JJ*, Chen HY. Attomole antigen detection using self-electrochemiluminous graphene oxide-capped Au@ L012 nanocomposite. Anal. Chem. 2017, 89, 2418-2423.

34. Xu JJ, Jiang DP, Qin YL, Xia J, Jiang DC*, Chen HY. C₃N₄ nanosheet modified microwell array with enhanced electrochemiluminescence for total analysis of cholesterol at single cells. Anal. Chem. 2017, 89, 2216-2220.

33. Pan RR, Xu MC, Jiang DC*, Burgess JD, Chen HY. Nanokit for single-cell electrochemical analyses. Proc. Natl. Acad. Sci. U.S.A. 2016, 113, 11436-11440.

32. Yang CY, Qin Y, Jiang DC*, Chen HY. Continuous fluorescence imaging of intracellular calcium by use of ion-selective nanospheres with adjustable spectra. ACS Appl. Mater. Interfaces 2016, 8, 19892-19898.

31. Xu JJ, Huang PY, Qin Y, Jiang DC*, Chen HY. Analysis of intracellular glucose at single cells using electrochemiluminescence imaging. Anal. Chem. 2016, 88, 4609-4612.

30. Xu JJ, Zhai JY, Xu YM, Zhu JW, Qin Y*, Jiang DC*. A near-infrared fluorescent aza-bodipy probe for dual-wavelength detection of hydrogen peroxide in living cells. Analyst 2016, 141, 2380-2383.

29. He RQ, Tang HF, Jiang DC*, Chen HY. Electrochemical visualization of intracellular hydrogen peroxide at single cells. Anal. Chem. 2016, 88, 2006-2009.

28. Zhou JY, Ma GZ, Chen Y, Fang DJ*, Jiang DC*, Chen HY. Electrochemiluminescence imaging for parallel single-cell analysis of active membrane cholesterol. Anal. Chem. 2015, 87, 8138-8143.

27. Liu YL, Qin Y*, Jiang DC*. Squaramide-based tripodal ionophores for potentiometric sulfate-selective sensors with high selectivity. Analyst 2015, 140, 5317-5323.

26. Liu YL, Zhu JW, Xu YM, Qin Y*, Jiang DC*. Boronic acid functionalized aza-Bodipy (azaBDPBA) based fluorescence optodes for the analysis of glucose in whole blood. ACS Appl. Mater. Interfaces 2015, 7, 11141-11145.

25. Zhuang LH, Zuo HZ, Wu ZQ, Wang Y, Fang DJ*, Jiang D*. Enhanced electrochemical nanoring electrode for analysis of cytosol in single cells. Anal. Chem. 2014, 86, 11517-11522.

24. Han FF, Jiang H, Fang DJ*, Jiang DC*. Potential-resolved electrochemiluminescence for determination of two antigens at the cell surface. Anal. Chem. 2014, 86, 6896-6902.

23. Tian CX, Zhou JY, Wu ZQ, Fang DJ*, Jiang DC*. Fast serial analysis of active cholesterol at the plasma membrane in single cells. Anal. Chem. 2014, 86, 678-684.

22. Ma GZ, Zhou JY, Tian CX, Jiang DC*, Fang DJ*, Chen HY. Luminol electrochemiluminescence for the analysis of active cholesterol at the plasma membrane in single mammalian cells. Anal. Chem. 2013, 85, 3912-3917.

21. Han FF, Xu YM, Jiang DC*, Qin Y*, Chen HY. A BODIPY-derived fluorescent probe for cellular pH measurements. Anal. Biochem. 2013, 435, 106-113.

20. Wang KL, Jiang DC, Sims CE, Allbritton NL*. Separation of fluorescently labeled phosphoinositides and sphingolipids by capillary electrophoresis. J. Chromatogr. B 2012, 907, 79-86.

19. Huang WG, Jiang DC, Wang XY, Wang KL, Sims CE, Allbritton NL*, Zhang QS*. Kinetic analysis of PI3K reactions with fluorescent PIP2 derivatives. Anal. Bioanal. Chem. 2011, 401, 1881-1888.

18. Jiang DC, Sims CE, Allbritton NL*. Single-cell analysis of phosphoinositide 3-kinase and phosphatase and tensin homolog activation. Faraday Discuss. 2011, 149, 187-200.

17. Jiang DC, Sims CE, Allbritton NL*. Microelectrophoresis platform for fast serial analysis of single cells. Electrophoresis 2010, 31, 2558-2565.

16. Fang DJ, West RH, Manson ME, Ruddy J, Jiang DC, Previs SF, Sonawane ND, Burgess JD, Kelley TJ*. Increased plasma membrane cholesterol in cystic fibrosis cells correlates with CFTR genotype and depends on de novo cholesterol synthesis. Respir. Res. 2010, 11, 1-12.

15. Fang DJ, Jiang DC, Lu H, Chiel HJ, Kelley TJ, Burgess JD*. Observation of cellular cholesterol efflux at microcavity electrodes. J. Am. Chem. Soc. 2009, 131, 12038-12039.

14. Ding JX, Jiang DC, Kurczy M, Nalepka J, Dudley B, Merkel EI, Porter FD, Ewing AG, Winograd N, Burgess J, Molyneaux K*. Inhibition of HMG CoA reductase reveals an unexpected role for cholesterol during PGC migration in the mouse. BMC Dev. Biol. 2008, 8, 1-14.

13. Jiang DC, Fang DJ, Kelley TJ, Burgess JD*. Electrochemical analysis of cell plasma membrane cholesterol at the airway surface of mouse trachea. Anal. Chem. 2008, 80, 1235-1239.

12. Jiang DC, Devadoss A, Palencsár MS, Fang DJ, White NM, Kelley TJ, Smith JD, Burgess JD*. Direct electrochemical evaluation of plasma membrane cholesterol in live mammalian cells. J. Am. Chem. Soc. 2007, 129, 11352-11353.

11. White NM, Jiang DC, Burgess JD, Bederman IR, Previs SF, Kelley TJ*. Altered cholesterol homeostasis in cultured and in vivo models of cystic fibrosis. Am. J. Physiol-Lung Cell. Mol. Physiol. 2007, 292, L476-L486.

10. Devadoss A, Palencsár MS, Jiang DC, Honkonen ML, Burgess JD*. Enzyme modification of platinum microelectrodes for detection of cholesterol in vesicle lipid bilayer membranes. Anal. Chem. 2005, 77, 7393-7398.

9. Jiang DC, Tang J, Liu BH, Yang PY, Kong JL*. Ultrathin alumina sol-gel-derived films: allowing direct detection of the liver fibrosis markers by capacitance measurement. Anal. Chem. 2003, 75, 4578-4584.

8. Jiang DC, Tang J, Liu BH, Yang PY, Shen XR, Kong JL*. Covalently coupling the antibody on an amine-self-assembled gold surface to probe hyaluronan-binding protein with capacitance measurement. Biosens. Bioelectron. 2003, 18, 1183-1191.

7. Xu X, Zhao JQ, Jiang DC, Kong JL, Liu BH*, Deng JQ. TiO₂ sol-gel derived amperometric biosensor for H₂O₂ on the electropolymerized phenazine methosulfate modified electrode. Anal. Bioanal. Chem. 2002, 374, 1261-1266.

6. Wang K, Jiang DC, Liu BH, Zhang S, Lu TP, Kong JL*. Label-free immunosensors-principles and applications. Chinese J. Anal. Chem. 2005, 33, 411-416.

5. Liu Z, Huang S, Jiang DC, Liu BH, Kong JL*. A novel capacitive immunosensor using electropolymerized insulating poly (o‐phenylenediamine) film on a glass carbon electrode for probing transferrin. Anal. Lett. 2004, 37, 2283-2301.

4. Jiang DC, Huang S, Tang J, Liu BH, Huang YP, Kong JL*. A high sensitive hyaluronan binding protein capacitive immunosensor. Chinese J. Anal. Chem. 2003, 31, 713-715.

3. Wang K, Jiang DC, Kong JL, Zhang S, Liu BH*, Lu TP*. Sensitively detecting recombinant hirudin variant-2 with capacitive immunoassay based on self-assembled monolayers. Anal. Lett. 2003, 36, 2571-2583.

2. Yang HF, Shi QH, Liu XY, Xie SH, Jiang DP, Zhang FQ, Yu CZ, Tu B, Zhao DY*. Synthesis of ordered mesoporous carbon monoliths with bicontinuous cubic pore structure of Ia3d symmetry. Chem. Comm. 2002, 23, 2842-2843.

1. Zhang JR*, Jiang DC, Chen B, Zhu JJ, Jiang LP, Fang HQ. Preparation and electrochemistry of hydrous ruthenium oxide/active carbon electrode materials for supercapacitor. J. Electrochem. Soc. 2001, 148, A1362-A1367.

Post-Doc. Jingjing Zhang, Yu Li, Rongrong Pan

Ph.D students: Nina Wang, Yuling Wang, Rong Jin

M.S. students: Yucheng Huang, Rongcan Huang, Yinzi Qian, Xingqi Chang